Identification of Outer Membrane Vesicles Derived from Orientia tsutsugamushi
Journal of Korean Medical Science
;
: 866-870, 2015.
Article
in English
| WPRIM
| ID: wpr-210701
ABSTRACT
Orientia tsutsugamushi, a causative pathogen of Scrub typhus, is a gram-negative intracellular bacterium. Outer membrane vesicles (OMVs) are produced from the membrane of bacteria and play many roles related to the survival of the pathogen. However, there have been no reports confirming whether O. tsutsugamushi indeed produce OMVs. O. tsutsugamushi boryong was cultured in ECV-304 cells for the purification of OMVs. Western blot analysis and immunoenrichment using anti-O. tsutsugamushi monoclonal antibody and electron microscopy were employed for identification and characterization of OMVs. We confirm the presence of OMVs derived from O. tsutsugamushi, and also found that those OMVs contain a major surface antigen of 56-kDa protein and variant immunogenic antigens.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Orientia tsutsugamushi
/
Scrub Typhus
/
Microscopy, Electron
/
Cell Line
/
Cell Membrane
/
Secretory Vesicles
/
Antibodies, Monoclonal
/
Antigens, Bacterial
/
Antigens, Surface
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Language:
English
Journal:
Journal of Korean Medical Science
Year:
2015
Type:
Article
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