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Clinical application of preimplantation genetic diagnosis (PGD) using fluorescence in-situ hybridization to balanced reciprocal or Robertsonian translocation carriers in human IVF-ET program / 대한산부인과학회잡지
Article in Korean | WPRIM | ID: wpr-210840
Responsible library: WPRO
ABSTRACT

OBJECTIVE:

This study was performed to evaluate the efficiency of preimplantation genetic diagnosis (PGD) using fluorescence in-situ hybridization (FISH) in Robertsonian or balanced reciprocal translocation carriers in human IVF-ET programm.

METHOD:

FISH was carried out in 25 cycles of 15 couples. Two-color FISH analysis was performed on 54 polar bodies in 3 cycles and 234 blastomeres in 22 cycles. After FISH analysis, the embryos with normal FISH signals were transferred into mother's uterus.

RESULTS:

In FISH analysis of polar bodies, 18 nuclei of polar bodies were normal and 12 embryos were transferred in 3 cycles. FISH efficiency per oocyte was 95.0% in cases using polar bodies. In FISH analysis of blastomeres, 49 embryos were normal and transferred in 21 cycles. FISH efficiency per embryo was 92.7% using blastomeres. At present, three pregnancies were achieved. A girl and a boy were delivered. Both of them were translocation carriers. The other conceptus showed normal karyotype.

CONCLUSIONS:

According to this study, PGD using FISH can be successfully applied for the patients with translocations of chromosomes.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Oocytes / Prostaglandins D / Uterus / Blastomeres / Female / Humans / Male / Pregnancy / Family Characteristics / Preimplantation Diagnosis Language: Korean Journal: Korean Journal of Obstetrics and Gynecology Year: 2000 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Oocytes / Prostaglandins D / Uterus / Blastomeres / Female / Humans / Male / Pregnancy / Family Characteristics / Preimplantation Diagnosis Language: Korean Journal: Korean Journal of Obstetrics and Gynecology Year: 2000 Type: Article