Clinical application of preimplantation genetic diagnosis (PGD) using fluorescence in-situ hybridization to balanced reciprocal or Robertsonian translocation carriers in human IVF-ET program / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 1147-1153, 2000.
Article
in Korean
| WPRIM
| ID: wpr-210840
ABSTRACT
OBJECTIVE:
This study was performed to evaluate the efficiency of preimplantation genetic diagnosis (PGD) using fluorescence in-situ hybridization (FISH) in Robertsonian or balanced reciprocal translocation carriers in human IVF-ET programm.METHOD:
FISH was carried out in 25 cycles of 15 couples. Two-color FISH analysis was performed on 54 polar bodies in 3 cycles and 234 blastomeres in 22 cycles. After FISH analysis, the embryos with normal FISH signals were transferred into mother's uterus.RESULTS:
In FISH analysis of polar bodies, 18 nuclei of polar bodies were normal and 12 embryos were transferred in 3 cycles. FISH efficiency per oocyte was 95.0% in cases using polar bodies. In FISH analysis of blastomeres, 49 embryos were normal and transferred in 21 cycles. FISH efficiency per embryo was 92.7% using blastomeres. At present, three pregnancies were achieved. A girl and a boy were delivered. Both of them were translocation carriers. The other conceptus showed normal karyotype.CONCLUSIONS:
According to this study, PGD using FISH can be successfully applied for the patients with translocations of chromosomes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oocytes
/
Prostaglandins D
/
Uterus
/
Blastomeres
/
Family Characteristics
/
Preimplantation Diagnosis
/
Embryonic Structures
/
Polar Bodies
/
Karyotype
/
Fluorescence
Type of study:
Diagnostic study
Limits:
Female
/
Humans
/
Male
/
Pregnancy
Language:
Korean
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2000
Type:
Article
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