Enhanced tyrosine hydroxylase expression in PC12 cells co-cultured with feline mesenchymal stem cells
Journal of Veterinary Science
;
: 377-382, 2007.
Article
in English
| WPRIM
| ID: wpr-210999
ABSTRACT
Mesenchymal stem cells (MSCs) secrete a variety of neuroregulatory molecules, such as nerve growth factor, brain-derived neurotrophic factor, and glial cell-derived neurotrophic factor, which upregulate tyrosine hydroxylase (TH) gene expression in PC12 cells. Enhancing TH gene expression is a critical step for treatment of Parkinson's disease (PD). The objective of this study was to assess the effects of co-culturing PC12 cells with MSCs from feline bone marrow on TH protein expression. We divided the study into three groups an MSC group, a PC12 cell group, and the combined MSC + PC12 cell group (the co-culture group). All cells were cultured in DMEM-HG medium supplemented with 10% fetal bovine serum for three days. Thereafter, the cells were examined using western blot analysis and immunocytochemistry. In western blots, the co-culture group demonstrated a stronger signal at 60 kDa than the PC12 cell group (p < 0.001). TH was not expressed in the MSC group, either in western blot or immunocytochemistry. Thus, the MSCs of feline bone marrow can up-regulate TH expression in PC12 cells. This implies a new role for MSCs in the neurodegenerative disease process.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tyrosine 3-Monooxygenase
/
Immunohistochemistry
/
Cats
/
Gene Expression Regulation, Enzymologic
/
Cells, Cultured
/
Blotting, Western
/
Microscopy, Phase-Contrast
/
PC12 Cells
/
Cell Culture Techniques
/
Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2007
Type:
Article
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