The dose of cyclophosphamide for treating paraquat-induced rat lung injury
The Korean Journal of Internal Medicine
; : 420-427, 2013.
Article
in En
| WPRIM
| ID: wpr-212582
Responsible library:
WPRO
ABSTRACT
BACKGROUND/AIMS: Cyclophosphamide (CP) is a promising treatment for severe cases of paraquat (PQ) poisoning. We investigated the effective dose of CP for mitigating PQ-induced lung injury. METHODS: Adult male Sprague-Dawley rats were allocated into five groups: control, PQ (35 mg/kg, intraperitoneal injection), and PQ + CP (1.5, 15, or 30 mg/kg). The dimensions of lung lesions were determined using X-ray microtomography (micro-CT), and histological changes and cytokine levels were recorded. RESULTS: The micro-CT results showed that 15 mg/kg CP was more effective than 1.5 mg/kg CP for treating PQ-induced lung injury. At a dose of 1.5 mg/kg, CP alleviated the histological evidence of inflammation and altered superoxide dismutase activity. Using 15 mg/kg CP reduced the elevated catalase activity and serum transforming growth factor (TGF)-beta1 level. CONCLUSIONS: A CP dose of > 15 mg/kg is effective for reducing the severity of PQ-induced lung injury as determined by histological and micro-CT tissue examination, possibly by modulating antioxidant enzyme and TGF-beta1 levels.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Paraquat
/
Pulmonary Edema
/
Superoxide Dismutase
/
Severity of Illness Index
/
Catalase
/
Cytokines
/
Rats, Sprague-Dawley
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Oxidative Stress
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Inflammation Mediators
/
Cyclophosphamide
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
The Korean Journal of Internal Medicine
Year:
2013
Type:
Article