Oxidative Damage induced by UV in Cultured Retinal Pigment Epithelial Cells
Journal of the Korean Ophthalmological Society
;
: 2890-2895, 2003.
Article
in Korean
| WPRIM
| ID: wpr-212683
ABSTRACT
PURPOSE:
The death of retinal pigment epithelial(RPE) cells plays an important role in the development of age-related macular degeneration. We studied whether ultraviolet could induce apoptotic cell death and the increase of reactive oxygen intermediates (ROI) in the RPE cells.METHODS:
Cultured human RPE cells were exposed to doses of between 0 and 80 mJ/cm2 UVB as an attached monolayer. The number of viable cells was assessed by trypan blue assay. The apoptosis was determined by flow cytometry using annexin V and propidium iodide (PI). Intracellular ROI was measured using DCFH-DA.RESULTS:
UVB-exposed cells showed a dose-dependent cell death. The number of cells decreased in 20~30% at the dose of 40 mJ/cm2 UVB 24 hours after UVB exposure. The proportion of annexin V positive cells and intracellular ROI increased after UVB exposure.CONCLUSIONS:
These results suggest that UV-induced oxidative damage may be one of the important mechanisms of RPE cell death.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxygen
/
Propidium
/
Retinaldehyde
/
Trypan Blue
/
Cell Death
/
Apoptosis
/
Annexin A5
/
Epithelial Cells
/
Retinal Pigment Epithelium
/
Flow Cytometry
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2003
Type:
Article
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