Effects of Glutathione on Cisplatin-Induced Cytotoxicity In Human Cervical Cancer Cell Lines / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 1289-1293, 2002.
Article
in Korean
| WPRIM
| ID: wpr-213768
ABSTRACT
OBJECTIVE:
The purpose of this study is to determine the effects of glutathione on cisplatin-induced cytotoxicity of human cervical carcinoma cell lines (SiHa squamous cell carcinoma cell, CaSki epidermoid metastatic carcinoma cell).METHODS:
Human cervical carcinoma cells (SiHa, CaSki) were incubated with culture media (RPMI1640) in the presence of cisplatin and/or buthionine sulfoximine (BSO), as a inhibitor of gamma-glutamyl- cysteine synthetase, and/or glutathione (GSH) and/or 2-oxo 4-thiazolidine carboxylic acid (OTC). The viable cells were examined by using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) and was determined by spectrophotometer at 570 nm.RESULTS:
The incubation of cervical cells with cisplatin resulted in an decreasing cells viability by dose response. The MTT reduction rate were not different by BSO (5 mM) treatment in cervical cell lines. The viable cells were increased significantly by glutathione (5 mM) or OTC (5 mM) in cisplatin-treated cell lines.CONCLUSION:
gamma-glutamylcysteine synthetase inhibitor had no effect on cisplatin toxicity. GSH and OTC had effect on cisplatin cytotoxicity. So, These result suggested that cervical cancer line cells were more susceptive to protective effects of glutathione and OTC than BSO on cisplatin induced-toxicity.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Carcinoma, Squamous Cell
/
Cell Line
/
Uterine Cervical Neoplasms
/
Cisplatin
/
Buthionine Sulfoximine
/
Culture Media
/
Cysteine
/
Glutathione
/
Ligases
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2002
Type:
Article
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