A New Rapid and Non-radioactive Assay for Monitoring and Determining the Proliferation of Retinal Pigment Epithelial Cells
Korean Journal of Ophthalmology
; : 29-34, 2003.
Article
in En
| WPRIM
| ID: wpr-213883
Responsible library:
WPRO
ABSTRACT
AlamarBlue is used to induce color and fluorescence in the microenvironment of activated cells. The alamarBlue assay was studied to determine if it could be used as a method of evaluating the number of retinal pigment epithelial (RPE) cells. A series of two-fold dilutions of RPE cells were placed into 96-well culture plates. The alamarBlue was added to the culture media after attaching the cells. The absorbance and fluorescence were measured consecutively at various intervals over a period of 24 hr. Cell viability were evaluated by means of the trypan blue exclusion method and flow cytometry using a combination of propidium iodide and annexin V was done to prove the safety of alamarBlue assay to the cells. Both the absorbance and the fluorescence had a linear relationship with the number of RPE cells. Exposing the RPE cells to alamarBlue was not detrimental to the cells. In conclusion, the alamarBlue assay constitutes a one-step, extremely simple, reproducible, economical and non-toxic procedure for evaluating the number of viable RPE cells.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Pigment Epithelium of Eye
/
Trypan Blue
/
Cell Count
/
Cell Division
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Cell Survival
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Coloring Agents
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Flow Cytometry
/
Fluorescence
Limits:
Humans
Language:
En
Journal:
Korean Journal of Ophthalmology
Year:
2003
Type:
Article