Effects of Interleukin-4 on Extracellular Matrix Gene Expression in Normal Skin Fibroblasts Cultures
Annals of Dermatology
;
: 90-97, 1996.
Article
in English
| WPRIM
| ID: wpr-214262
ABSTRACT
BACKGROUND:
Interleukin-4(IL-4) is a 20 kDa glycoprotein and is now known to possess fibrogenic activities.OBJECTIVE:
The purpose of this study is to evaluate the effect of IL4 on the extracellular matrix gene expression.METHODS:
Quantitation of collagenous protein synthesis, Northern and dot-blot hybridization, transfection experiments and CAT assay in normal human skin fibroblasts were done.RESULTS:
Maximal elevation of collagen synthesis was presented at the concentration of IL-4 being S.Ong/ml. In Northern and dot-blot analysis, each level of type I collagens and fibronectin mRNA increased 3.0, and 2.8-fold, respectively in IL4 treated fibroblasts. In CAT to c assay, the percentage of acetylation was 8.3% in the untreated control group and 23.1% in 5.Ong/ml of the IL-4 treated group in normal fibroblasts. The promoter activity was in creased 2.8-fold in II.4(5.Ong/ml) treated fibroblasts compared to the control groups.CONCLUSION:
IL4 might be a fibrogenic cytokine that could be important in promoting the biogenesis of collagen proteins. This could be due to increased transcription.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Acetylation
/
Skin
/
RNA, Messenger
/
Glycoproteins
/
Transfection
/
Gene Expression
/
Collagen
/
Fibronectins
/
Interleukin-4
/
Collagen Type I
Limits:
Animals
/
Humans
Language:
English
Journal:
Annals of Dermatology
Year:
1996
Type:
Article
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