Th1/Th2 Cytokine Production in Patients with Chronic Hepatitis C Virus Infection / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 246-252, 2002.
Article
in Korean
| WPRIM
| ID: wpr-214318
ABSTRACT
BACKGROUND:
To evaluate the immunoregulatory mechanism of Th1/Th2 cells in patients with the Hepatitis C virus infection, which is characterized by persistence and chronic progression, Th1 cytokine (IFN-, IL-12, IL-2) and Th2 cytokine (IL-4) responses were investigated.METHODS:
Th1 cytokine (IFN-, IL-12, and IL-2) and Th2 cytokine (IL-4) for serum levels, spontaneous production of peripheral mononuclear cells (PBMC) and PHA-stimulated production of PBMC were estimated by the ELISA method in 19 patients with chronic Hepatitis C (CH), 12 with cirrhosis of the liver (LC) and 12 healthy subjects.RESULTS:
The IFN-gamma serum levels increased significantly in the CH and LC compared with the healthy controls (P<0.05). The spontaneous IFN-gamma production of PBMC increased significantly in patients with CH and LC compared with the healthy controls (P<0.05, P<0.01, respectively). The PHA stimulated IFN-gamma production of PBMC decreased significantly in chronic liver disease (CLD) compared with the healthy controls according to production ability. The IL-12 for serum and spontaneous IL-12 production of PBMC fell under the detectable level. PHA-stimulated IL-12 production decreased significantly in the CH compared with the healthy controls (P<0.01). The IFN-gamma showed positive correlations with the lymphocyte counts and IL-12 levels in CLD.CONCLUSIONS:
Although the Th1 immune response for serum and spontaneous production in CH and LC was normal or increased, the Th1 immune response for PHA stimulation decreased or showed a low-tendency. It is suggested that the cytokine imbalance in the CLD may contribute to the persistence of HCV infection; however, it was not correlated with chronic progression.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Fibrosis
/
Enzyme-Linked Immunosorbent Assay
/
Hepacivirus
/
Lymphocyte Count
/
Interleukin-12
/
Hepatitis C, Chronic
/
Hepatitis, Chronic
/
Liver
/
Liver Diseases
Limits:
Humans
Language:
Korean
Journal:
The Korean Journal of Laboratory Medicine
Year:
2002
Type:
Article
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