Genetic Variation in Exon 3 of Human Apo B mRNA Editing Protein (apobec-1) Gene
Journal of Genetic Medicine
;
: 15-20, 1999.
Article
in English
| WPRIM
| ID: wpr-214517
ABSTRACT
We have investigated the genetic variation in the human apo B mRNA editing protein (apobec-1) gene. Exon 3 of the apobec-1 gene was amplified by polymerase chain reaction. After detection of an additional band by single strand conformational polymorphism (SSCP) analysis, sequencing of the SSCP shift sample revealed a single-base mutation. The mutation was a CGG transversion at codon 80 resulting in a lleRMet substitution. Thes substitution was confirmed by restriction fragment length polymorphism analysis since a pvull site is abolished by the substitution. Population and family studies confirmed that the inheritance of the genotypes for apobec-1 gene polyomrphism is comtrolled by two codominant alleles (P1 and P2). A significant difference in plasma triglyceride was detected among the different apobec-1 genotypes in the CAD patients (P<0.05) Our study could provide the basis for elucidating the interaction between gentic variation of the apobec-1 gene and disorders related to lipid metabolism.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Apolipoproteins B
/
Plasma
/
Triglycerides
/
Genetic Variation
/
Wills
/
Polymorphism, Restriction Fragment Length
/
Codon
/
RNA, Messenger
/
Polymerase Chain Reaction
/
Exons
Limits:
Humans
Language:
English
Journal:
Journal of Genetic Medicine
Year:
1999
Type:
Article
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