The evaluation of periodontal ligament cells of rat teeth after low-temperature preservation under high pressure / 대한치과보존학회지
Journal of Korean Academy of Conservative Dentistry
;
: 285-294, 2010.
Article
in Korean
| WPRIM
| ID: wpr-214634
ABSTRACT
The purpose of this study was to evaluate the viability of periodontal ligament cells of rat teeth after low-temperature preservation under high pressure by means of MTT assay, WST-1 assay. 12 teeth of Sprague-Dawley white female rats of 4 week-old were used for each group. Both side of the first and second maxillary molars were extracted as atraumatically as possible under tiletamine anesthesia. The experimental groups were group 1 (Immediate extraction), group 2 (Slow freezing under pressure of 3 MPa), group 3 (Slow freezing under pressure of 2 MPa), group 4 (Slow freezing under no additional pressure), group 5 (Rapid freezing in liquid nitrogen under pressure of 2 MPa), group 6 (Rapid freezing in liquid nitrogen under no additional pressure), group 7 (low-temperature preservation at 0degrees C under pressure of 2 MPa), group 8 (low-temperature preservation at 0degrees C under no additional pressure), group 9 (low-temperature preservation at -5degrees C under pressure of 90 MPa). F-medium and 10% DMSO were used as preservation medium and cryo-protectant. For cryo-preservation groups, thawing was performed in 37degrees C water bath, then MTT assay, WST-1 assay were processed. One way ANOVA and Tukey HSD method were performed at the 95% level of confidence. The values of optical density obtained by MTT assay and WST-1 were divided by the values of eosin staining for tissue volume standardization. In both MTT and WST-1 assay, group 7 (0degrees C/2 MPa) showed higher viability of periodontal ligament cells than other group (2-6, 8) and this was statistically significant (p < 0.05), but showed lower viability than group 1, immediate extraction group (no statistical significance). By the results of this study, low-temperature preservation at 0degrees C under pressure of 2 MPa suggest the possibility for long term preservation of teeth.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Periodontal Ligament
/
Tiletamine
/
Tooth
/
Baths
/
Cryopreservation
/
Dimethyl Sulfoxide
/
Eosine Yellowish-(YS)
/
Freezing
/
Anesthesia
/
Molar
Limits:
Animals
/
Female
/
Humans
Language:
Korean
Journal:
Journal of Korean Academy of Conservative Dentistry
Year:
2010
Type:
Article
Similar
MEDLINE
...
LILACS
LIS