The Effect of Chitosan-TGF-beta1 Conjugate on Full Thickness Articular Cartilage Defect in the Rabbit Knee Model (Preliminary Report) / 대한정형외과연구학회지
Journal of Korean Orthopaedic Research Society
;
: 58-67, 2005.
Article
in Korean
| WPRIM
| ID: wpr-214787
ABSTRACT
PURPOSE:
To evaluate the effect of the chitosan-TGF-beta1 conjugate on articular cartilage defects of rabbits' knees MATERIALS ANDMETHODS:
Full thickness cartilage defect(6mm, round shape) was made at both knees of 20 rabbits and, after the lapse of 3 days, chitosan-TGF-beta1(15ng/ml, 1ml) was injected into one side(experimental group) of knees and PBS(1ml) was injected into the other side(control group). 5 rabbits were sacrificed 6 weeks after the injection and the rest of 15 rabbits 12 weeks later. Then, gross morphology and histological evaluation(Mendelson scoring) was conducted.RESULTS:
No arthritic findings was observed and histological results in the experimental group at 6(Ave. 11.3+/-1.5) and 12(Ave. 4.5+/-1.9) weeks postoperatively were superior to those in the control group at 6(Ave. 14.6+/-1.7) and 12(Ave. 9.8+/-2.2) weeks. Especially at 12 weeks, the experimental group was superior to the control group statistically in results of 5 subgroups except for filling of defects. The difference of two groups at 12 weeks was more remarkable than those at 6 weeks.CONCLUSION:
Regeneration of something very close to normal cartilage was observed in the experimental group. It shows that the biological activity of TGF-beta1 is sustained by the action of conjugate with chitosan, through prolonged half life of TGF-beta1.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Regeneration
/
Cartilage
/
Cartilage, Articular
/
Chitosan
/
Transforming Growth Factor beta1
/
Half-Life
/
Knee
Type of study:
Prognostic study
Limits:
Animals
Language:
Korean
Journal:
Journal of Korean Orthopaedic Research Society
Year:
2005
Type:
Article
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