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Human Muscle Derived Stem Cell Differentiate into Neurons / 대한정형외과연구학회지
Journal of Korean Orthopaedic Research Society ; : 41-49, 2005.
Article in Korean | WPRIM | ID: wpr-214789
ABSTRACT

PURPOSE:

To evaluate the possibility that human muscle derived stem cells (hMDSCs) can be differentiated into neurons in vitro. MATERIAL AND

METHODS:

Muscle derived stem cells were isolated from the hamstring muscles during the anterior cruciate ligament reconstruction by preplate technique. For the characterization of these cells, desmin staining, CD 34, Sca-1, CD 29 using the Flow cytometry were performed. In the experimental group, neuronal induction media was added to differentiate hMDSCs to neuronal cells. These cells were evaluated by neuronal markers such as neuron-specific enolase (NSE), neurofilament (NF), TrkA using immunocytochemistry. For the control group, no induction media was added. Statstical analyses were performed by use of Kruskal-Wallis H test and Student-Newman-Keuls test (P<0.01).

RESULT:

Desmin staining was positive in 92.3+/-6%. Flow cytometry was negative for CD 34 and Sca-1. However it was positive for CD 29. (69.4+/-10%). The immunocytochemical result revealed NSE, NF and TrkA positive with 63.2+/-2.3%, 59.2+/-2.5%, 55+/-2.4% respectively. However, these were negative in the control group.

CONCLUSION:

Our observations indicate that hMDSCs have the capacity to differentiate into neurons in a specialized culture media.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Phosphopyruvate Hydratase / Stem Cells / Immunohistochemistry / Culture Media / Desmin / Anterior Cruciate Ligament Reconstruction / Flow Cytometry / Muscles / Neurons Limits: Humans Language: Korean Journal: Journal of Korean Orthopaedic Research Society Year: 2005 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phosphopyruvate Hydratase / Stem Cells / Immunohistochemistry / Culture Media / Desmin / Anterior Cruciate Ligament Reconstruction / Flow Cytometry / Muscles / Neurons Limits: Humans Language: Korean Journal: Journal of Korean Orthopaedic Research Society Year: 2005 Type: Article