Paracrine Effects of Adipose-Derived Stem Cells on Keratinocytes and Dermal Fibroblasts
Annals of Dermatology
;
: 136-143, 2012.
Article
in English
| WPRIM
| ID: wpr-214981
ABSTRACT
BACKGROUND:
Adipose-derived stem cells (ASCs) are mesenchymal stem cells that have recently been applied to tissue repair and regeneration. Keratinocytes and dermal fibroblasts play key roles in cutaneous wound healing.OBJECTIVE:
We investigated the paracrine effects of ASCs on HaCaT cells (i.e., immortalized human keratinocytes) and human dermal fibroblasts to explore the mechanism of the effects of ASCs on cutaneous wound healing.METHODS:
HaCaT cells and primary cultured human dermal fibroblasts were treated with 50% conditioned medium of ASCs (ASC-CM). Viability, in vitro wound healing, and fibroblast-populated collagen lattice contraction assays were conducted, and reverse transcription-polymerase chain reaction (RT-PCR) for the type I procollagen alpha1 chain gene was performed.RESULTS:
The proliferation of HaCaT cells and fibroblasts was increased by ASC-CM in the viability assay. ASC-CM promoted in vitro wound healing of HaCaT cells and increased the contraction of the fibroblast-populated collagen lattice. RT-PCR showed that the transcription of the type I procollagen alpha1 chain gene in fibroblasts was upregulated by ASC-CM.CONCLUSION:
The stimulatory effect of ASC on cutaneous wound healing may be partially mediated by paracrine effects of ASCs on other skin cells. Application of ASCs or ASC-derived molecules could be an innovative therapeutic approach in the treatment of chronic wounds and other conditions.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Regeneration
/
Skin
/
Stem Cells
/
Wound Healing
/
Keratinocytes
/
Adipose Tissue
/
Collagen
/
Culture Media, Conditioned
/
Collagen Type I
/
Contracts
Limits:
Humans
Language:
English
Journal:
Annals of Dermatology
Year:
2012
Type:
Article
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