Biochemical Analysis of the Changes of Collagen Types following Bladder Outlet Obstruction in the Rat / 대한비뇨기과학회지
Korean Journal of Urology
; : 723-728, 1998.
Article
in Ko
| WPRIM
| ID: wpr-215380
Responsible library:
WPRO
ABSTRACT
PURPOSE: We observed the ratio of collagen type III to type I by biochemical analysis in the adult rat bladder with chronic partial bladder outlet obstruction. MATERIALS AND METHODS: Female adult(20-wk-old, 200-250gm) Sprague-Dawley rats were divided into 3 groups: control group. 6-wk obstruction group, and 12-wk-obstruction group(n=7 in each group), Collagen samples, extracted from the whole bladders by pepsin digestion followed by salt precipitation, were analysed by SDS-PAGE on 5% or 8% polyacrylamide slab gels with in situ reduction or pre-reduction. Collagen polypeptides were wisualized by Coomassie blue staining and quantitated with a scanning densitometer. Collagen types were confirmed y Western blots. RESULTS: The ratio of collagen type III to type I increased gradually alona the obstructed period and the highest value was found in 12-wk-obstruction group(sontrol 0.29+/-0.023, 6-wk-obstruction 0.332+/-0.026, and 12-wk-obstruction0.395+/-0.047, mean+/- standard error). This change was statistically significant in the 12-wk-obstruction group compared to that of control. (p<0.05; by Wilcoxon signed rank test) CONCLUSIONS: Increasing type III collagen compared to type I collagen is a unique feature in the obstructed bladder.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Peptides
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Urinary Bladder
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Urinary Bladder Neck Obstruction
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Blotting, Western
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Pepsin A
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Collagen
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Rats, Sprague-Dawley
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Collagen Type I
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Collagen Type III
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Digestion
Limits:
Animals
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Female
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Humans
Language:
Ko
Journal:
Korean Journal of Urology
Year:
1998
Type:
Article