Mutational Analysis of Mitochondria DNA in Children with IgA Nephropathy
Journal of the Korean Society of Pediatric Nephrology
;
: 73-79, 2012.
Article
in Korean
| WPRIM
| ID: wpr-215814
ABSTRACT
PURPOSE:
The association of mitochondrial DNA (mtDNA) mutations, deletions and copy number with progressive changes in patients with some glomerular disease and end-stage renal disease have been reported. In this study, we performed mtDNA mutation analysis in children with IgA nephropathy to investigate its role in progressive clinical course.METHODS:
Seven children with IgA nephropathy were involved in this study. MtDNA isolated from platelet was amplified by PCR and sequenced entirely.RESULTS:
The mean age at renal biopsy was 11.5+/-2.2 year and the mean age at latest evaluation was 17.9+/-3.2 year. The mean follow-up period were 7.8+/-3.1 years. Patients was divided into 2 groups according to the amount of proteinuria at presenting manifestation. Group 2 patients were nephrotic syndrome. Renal function reveals within normal range in all patients. In group 2 patients, the mean serum albumin level was significantly lower than those of group 1 (3.7+/-0.6 g/dL vs. 4.7+/-0.2 g/dL, P=0.0241) and the mean total cholesterol level was significantly higher than those of group 1 (222.7+/-35.7 mg/dL vs. 148.3+/-29.1 mg/dL, P=0.0283). In Group 2 patients, total amount of protein of 24 hour collected urine also significantly higher than those of group 1 (1,466.0+/-742.5 mg vs. 122.5+/-48.1 mg, P=0.0135). Pr/Cr ratio in random urine sample was also higher in group 2 than those of group 1 but the statistical significance was not noted (1.8+/-1.6 vs. 0.2+/-0.2, P=0.0961). Deletion of mtDNA nt 8272-8281 were observed in two patients, one patient in each groups, respectively. This is non-coding lesion. No patients demonstrated the mtDNA mutations.CONCLUSIONS:
We have identified a deletion of mtDNA nt 8272-8281 in two children with IgA nephropathy. Further studies are needed to clarify the role of mitochondrial function in the progressive change of IgA nephropathy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Proteinuria
/
Reference Values
/
Biopsy
/
Blood Platelets
/
DNA
/
DNA, Mitochondrial
/
Immunoglobulin A
/
Serum Albumin
/
Polymerase Chain Reaction
/
Cholesterol
Type of study:
Observational study
/
Prognostic study
/
Risk factors
Limits:
Child
/
Humans
Language:
Korean
Journal:
Journal of the Korean Society of Pediatric Nephrology
Year:
2012
Type:
Article
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