Bioluminescence Imaging of Chondrocytes in Rabbits by Intraarticular Injection of D-Luciferin / 핵의학분자영상
Nuclear Medicine and Molecular Imaging
;
: 54-58, 2007.
Article
in Korean
| WPRIM
| ID: wpr-216027
ABSTRACT
PURPOSE:
Luciferase is one of the most commonly used reporter enzymes in the field of in vivo optical imaging. D-luciferin, the substrate for firefly luciferase has very high cost that allows this kind of experiment limited to small animals such as mice and rats. In this current study, we validated local injection of D-luciferin in the articular capsule for bioluminescence imaging in rabbits. MATERIALS ANDMETHODS:
Chondrocytes were cultured and infected by replication-defective adenoviral vector encoding firefly luciferase (Fluc). Chondrocytes expressing Fluc were injected or implanted in the left knee joint. The rabbits underwent optical imaging studies after local injection of D-luciferin at 1, 5, 7, 9 days after cellular administration. We sought whether optimal imaging signals was could be by a cooled CCD camera after local injection of D-luciferin.RESULTS:
Imaging signal was not observed from the left knee joint after intraperitoneal injection of D-luciferin (15 mg/kg), whereas it was observed after intraarticular injection. Photon intensity from the left knee joint of rabbits was compared between cell injected and implanted groups after intraarticular injection of D-luciferin. During the period of imaging studies, photon intensity of the cell implanted group was 5-10 times higher than that of the cell injected group.CONCLUSION:
We successfully imaged chondrocytes expressing Fluc after intraarticular injection of D-luciferin. This technique may be further applied to develop new drugs for knee joint disease.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Adenoviridae
/
Joint Capsule
/
Chondrocytes
/
Fireflies
/
Optical Imaging
/
Injections, Intra-Articular
/
Injections, Intraperitoneal
/
Knee Joint
/
Luciferases
Limits:
Animals
Language:
Korean
Journal:
Nuclear Medicine and Molecular Imaging
Year:
2007
Type:
Article
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