Usefulness of Immunohistochemistry for Microsatellite Instability Screening in Gastric Cancer
Gut and Liver
;
: 629-635, 2015.
Article
in English
| WPRIM
| ID: wpr-216107
ABSTRACT
BACKGROUND/AIMS:
The usefulness of immunohistochemistry to screen for the microsatellite instability (MSI) phenotype in gastric cancer remains unclear. Moreover, the prognostic value of MSI phenotypes in gastric cancer has been debated.METHODS:
The clinicopathologic parameters and survival outcomes of 203 MSI-high (MSI-H) and 261 microsatellite-stable (MSS) advanced gastric cancers (AGCs) were compared. Next, we compared the immunohistochemistry results for hMLH1 and hMSH2 with those of a polymerase chain reaction (PCR)-based method. Kaplan-Meier curves and a Cox proportional hazard regression model were used to conduct survival analyses.RESULTS:
The MSI-H AGCs were correlated with older age (p<0.001), female gender (p=0.018), distal location (p<0.001), larger size (p=0.016), and intestinal type (p<0.001). Multivariate analysis revealed that the MSI-H phenotype was an independent favorable factor that was related to overall survival in patients with AGC (p<0.001). Compared with the PCR-based analysis, immunohistochemistry exhibited high sensitivity (91.1%) and specificity (98.5%) in the detection of MSI phenotypes.CONCLUSIONS:
MSI-H gastric cancers have distinct clinicopathologic features and better prognoses, which suggests the necessity of MSI analysis in gastric cancer. Immunohistochemistry can be a useful and reliable screening method in the assessment of MSI status in gastric cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenotype
/
Prognosis
/
Stomach Neoplasms
/
Immunohistochemistry
/
Proportional Hazards Models
/
Sex Factors
/
Polymerase Chain Reaction
/
Predictive Value of Tests
/
Sensitivity and Specificity
/
Microsatellite Instability
Type of study:
Diagnostic study
/
Prognostic study
/
Screening study
Limits:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
Language:
English
Journal:
Gut and Liver
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS