Kinetic Analysis of Na-K-ATPase Activity of Rabbit Cerebral Cortex by DDVP
Journal of Korean Neurosurgical Society
;
: 17-22, 1982.
Article
in Korean
| WPRIM
| ID: wpr-21614
ABSTRACT
The kinetics of inhibition of Na-K-ATPase activity by o, 0-dimethyl o-(2,2-dichlorovinyl) phosphate(DDVP) was investigated with homogenerate of rabbit cerebral cortex. The results were summarized as the follows 1. Inhibition of Na-K-ATPase activity by DDVP showed a dose-dependent manner with an estimated I5.0 of 3.5x10(-4) M. 2. Altered pH and activity curves for Na-K-ATPase activity demonstrated comparable inhibition by DDVP in buffered acidic, neutral and alkaline pH renges. 3. Kinetic studies of cationic-substrate activation of Na-K-ATPase showed noncompetitive with respect to substrate and K+ and a mixed type inhibition with respect to Na+. These results suggest that DDVP appeared to exert its effects on Na-K-ATPase activity by interfering with the formation of Na-dependent phosphoenzyme.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Kinetics
/
Cerebral Cortex
/
Dichlorvos
/
Hydrogen-Ion Concentration
Language:
Korean
Journal:
Journal of Korean Neurosurgical Society
Year:
1982
Type:
Article
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