Lactoferrin Stimulates Mouse Macrophage to Express BAFF via Smad3 Pathway
Immune Network
;
: 84-88, 2012.
Article
in English
| WPRIM
| ID: wpr-216359
ABSTRACT
B cell-activating factor belonging to the TNF family (BAFF) is primarily expressed by macrophages and stimulates B cell proliferation, differentiation, survival, and Ig production. In this study, we explored the effect of lactoferrin (LF) on BAFF expression by murine macrophages. We determined the level of BAFF expression at the transcriptional and protein levels using RT-PCR and ELISA, respectively. LF markedly enhanced BAFF expression in mouse macrophages at both the transcriptional and protein levels. Overexpression of Smad3/4 further increased LF-induced BAFF transcription while DN-Smad3 abolished the LF-induced BAFF expression. These results demonstrate that LF can enhance BAFF expression through Smad3/4 pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Enzyme-Linked Immunosorbent Assay
/
Cell Proliferation
/
Transforming Growth Factor beta1
/
Lactoferrin
/
Macrophages
Limits:
Animals
/
Humans
Language:
English
Journal:
Immune Network
Year:
2012
Type:
Article
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