Effects of Transglutaminase 2 Inhibition on Ventilator-Induced Lung Injury
Journal of Korean Medical Science
; : 556-563, 2014.
Article
in En
| WPRIM
| ID: wpr-216480
Responsible library:
WPRO
ABSTRACT
This study was performed to examine the role of transglutaminase 2 (TG2) in ventilator-induced lung injury (VILI). C57BL/6 mice were divided into six experimental groups: 1) control group; 2) lipopolysaccharide (LPS) group; 3) lung protective ventilation (LPV) group; 4) VILI group; 5) VILI with cystamine, a TG2 inhibitor, pretreatment (Cyst+VILI) group; and 6) LPV with cystamine pretreatment (Cyst+LPV) group. Acute lung injury (ALI) score, TG2 activity and gene expression, inflammatory cytokines, and nuclear factor-kappaB (NF-kappaB) activity were measured. TG2 activity and gene expression were significantly increased in the VILI group (P < 0.05). Cystamine pretreatment significantly decreased TG2 activity and gene expression in the Cyst+VILI group (P < 0.05). Inflammatory cytokines were higher in the VILI group than in the LPS and LPV groups (P < 0.05), and significantly lower in the Cyst+VILI group than the VILI group (P < 0.05). NF-kappaB activity was increased in the VILI group compared with the LPS and LPV groups (P < 0.05), and significantly decreased in the Cyst+VILI group compared to the VILI group (P = 0.029). The ALI score of the Cyst+VILI group was lower than the VILI group, but the difference was not statistically significant (P = 0.105). These results suggest potential roles of TG2 in the pathogenesis of VILI.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Respiration, Artificial
/
Enzyme-Linked Immunosorbent Assay
/
Gene Expression
/
Transglutaminases
/
Lipopolysaccharides
/
Cytokines
/
NF-kappa B
/
GTP-Binding Proteins
/
Cystamine
/
Enzyme Inhibitors
Limits:
Animals
Language:
En
Journal:
Journal of Korean Medical Science
Year:
2014
Type:
Article