Prednisolone and Glucose-6-phosphatase Activity in Liver Cells
Yonsei Medical Journal
;
: 9-18, 1977.
Article
in English
| WPRIM
| ID: wpr-21669
ABSTRACT
Prednisolone, a cortisol analogue, was given intraperitoneally to rats with 5, 10 or 15 doses of 5 mg. per kg. of body weight per day. Sacrificing the animals 24 hours after the last injection, unfixed frozen sections from small pieces of liver tissue were incubated and stained by a modification from the method of Wachstein and Meisel(1965) for glucose-6-phosphatase (G-6-Pase) activity. Some of the tissue blocks were processed for staining with hematoxylin and eosin for histopathological observation. Glucose-6-phosphatase activity, being represented histochemically by brownish-black deposits, was progressively increased after administrations 5 or 10 times. With 15 doses of prednisolone the general histochemical picture of G-6-Pase activity appeared to be similar to that of the control group, except for a different distribution of hepatocytes possessing strong activity. In prednisolone treated rats, the swollen hepatocytes showed a marked, cytoplasmic vacuolization and nuclear pyknosis, particularly in the periportal and midzonal areas of hepatic lobules. Some discussion of the G-6-Pase corticosteroids are presented in terms of their metabolic effects.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Prednisolone
/
Glucose-6-Phosphatase
/
Liver
/
Animals
Limits:
Animals
Language:
English
Journal:
Yonsei Medical Journal
Year:
1977
Type:
Article
Similar
MEDLINE
...
LILACS
LIS