In Vivo Cartilage Formation Using Human Bone Marrow-Derived Mesenchymal Stem Cells Mixed with Fibrin Glue / 대한정형외과연구학회지
Journal of Korean Orthopaedic Research Society
;
: 43-50, 2015.
Article
in Korean
| WPRIM
| ID: wpr-217694
ABSTRACT
PURPOSE:
To investigate the feasibility of in vivo cartilage formation by direct injection the chondrogenic undifferentiated human bone marrow-derived mesenchymal stem cells (hBMSCs) mixed with fibrin glue including TGF-beta3. MATERIALS ANDMETHODS:
Chondrogenic differentiation induced hBMSCs for 14 days (control group-2) and undifferentiated hBMSCs combined with TGF-beta3 mixed (experimental group-3) with fibrin glue and fibrin glue only (control group-1) were injected subcutanteously into the back of nude mouse. For evaluation of the cartilage-like tissue formed after 8 weeks after injection, real time PCR, histological analysis and immunohistochemical analysis were used.RESULTS:
Control group-1 did not form any visible mass. Control group-2 as well as experimental group-3 could form new cartilage-like tissue which were demonstrated expression of type II collagen by real-time PCR, histology analysis such as H&E staining, MT staining and type II collagen specific immunohistologic analysis. As results, expression of type II collagen was shown in the both groups.CONCLUSION:
Our study confirmed that cartilage-like tissues could be formed in subcutaneous layer of nude mouse by direct injection mixed with fibrin glue including TGF-beta3 without chondrogenic-induction of hBMSCs, suggesting that these model could be suitable for preliminary studies or optimizing experiments to evaluate reconstruction of cartilage.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Fibrin
/
Cartilage
/
Fibrin Tissue Adhesive
/
Chondrogenesis
/
Collagen Type II
/
Transforming Growth Factor beta3
/
Mesenchymal Stem Cells
/
Real-Time Polymerase Chain Reaction
/
Mice, Nude
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Journal of Korean Orthopaedic Research Society
Year:
2015
Type:
Article
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