Histopathologic, Immunohistochemical, and Electron Microscopic Findings of Pigmented Skin Lesions Immediately after Q-switched Alexandrite Laser Treatment / 대한피부과학회지
Korean Journal of Dermatology
;
: 1014-1021, 2003.
Article
in Korean
| WPRIM
| ID: wpr-218225
ABSTRACT
BACKGROUND:
There have been only a few electron microscopic studies after laser treatment of pigmented skin lesions.OBJECTIVE:
The purpose was to investigate the pathologic, immunohistochemical, and electron microscopic changes following Q-switched alexandrite laser treatment of pigmented skin lesions.METHODS:
Three patients with acquired bilateral nevus of Ota-like macules, and 2 patients with cafeau lait macule were irradiated with Q-switched alexandrite laser. Forty biopsies were taken before and immediately after laser treatment. Hematoxylin-eosin, Fontana-Masson, and gp100 staining were performed for the evaluation of the histopathologic and immunohistochemical findings of the specimens. Electron microscopic findings were also evaluated.RESULTS:
Histopathologically, suprabasilar separations were observed immediately after laser treatment. Vacuolar alterations of pigment-containing cells were frequently found in the epidermis and/or in the dermis. Fontana-Masson and gp100 staining positivity changed to negative or decreased in the epidermis immediately after laser treatment, while they changed to negative in the dermis. Ultrastructurally, epidermal pigment-containing cells frequently showed severe vacuolar changes in the cytoplasm, pyknotic nuclei, and vacuolated and/or fragmented melanosomes immediately after laser treatment. Dermal melanocytes frequently revealed vacuolated and/or fragmented melanosomes immediately after laser treatment.CONCLUSION:
Histopathologic, immunohistochemical, and electron microscopic examination of pigmented skin lesions immediately after Q-switched alexandrite laser treatment demonstrated vacuolated or fragmented melanosomes and vacuolar alteration of pigment-containing cells in the epidermis and/or in the dermis, which suggested selective photothermolysis of melanosomes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Skin
/
Biopsy
/
Dermis
/
Melanosomes
/
Cytoplasm
/
Epidermis
/
Lasers, Solid-State
/
Melanocytes
/
Nevus
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Dermatology
Year:
2003
Type:
Article
Similar
MEDLINE
...
LILACS
LIS