Inhibitory effects of CTLA4-Ig fusion protein on the proliferation of T cell and the antibody production of B cell / 천식및알레르기
Journal of Asthma, Allergy and Clinical Immunology
;
: 818-825, 2003.
Article
in Korean
| WPRIM
| ID: wpr-218670
ABSTRACT
BACKGROUND:
Atopic asthma is characterized by activation of Th2-type T cells in the bronchial mucosa. Several reports have suggested an important role for costimulation through the CD28/CTLA4 (cytotoxic T lymphocyte-associated antigen 4)-B7 (CD80/CD86) pathway in allergen activation of T cells in animal models of allergen-induced asthma, because B7-CD28/ CTLA4 interaction can promote the differentiation and development of the Th2 lymphocyte subset.OBJECTIVE:
In the present study, we intended to investigate a potential role of humanized CTLA4-Ig on the inhibition of T and B cell activation by blocking B7/CD28 interactions.METHOD:
For this purpose we produced humanized CTLA4-Ig fusion protein by transfection to CHO cell and examined its inhibitory effects for activated T and B cell responses. We evaluated the inhibitory effect of MLR (mixed lymphocyte reaction) and con A-stimulated T cell proliferation. And we assayed wheather B cell was inhibited by stimulation of costimulatory signal in LPS-induced B cell response and PFC assay.RESULT:
In vitro assay, humanized CTLA4-Ig fusion protein inhibited T cell-specific immune response in dose-dependent manner CTLA4-Ig inhibited allogeneic stimulation in murine MLR, and the proliferation of T cell by the stimulation of Con A. But CTLA4-Ig did not inhibit directly the proliferative response of B cell by the stimulation of LPS. In addition, in vivo assay, CTLA4-Ig inhibited the production of antibody from B cell, which was presented by plaque-forming cell (PFC) assay.CONCLUSION:
These findings suggest that humanized CTLA4-Ig is effective to inhibit the proliferation of activated T cell directly by blocking B7/CD28 costimulation. And humanized CTLA4-Ig influences antibody-producing capacity of B cell indirectly by regulating T cell.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Asthma
/
Lymphocytes
/
T-Lymphocytes
/
Transfection
/
Lymphocyte Subsets
/
CHO Cells
/
Models, Animal
/
Cell Proliferation
/
Abatacept
/
Antibody Formation
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Journal of Asthma, Allergy and Clinical Immunology
Year:
2003
Type:
Article
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