Quantitation of T-lymphocyte Subsets Using the CELL-DYN Sapphire Automated Haematology Analyser / 임상검사와정도관리
Journal of Laboratory Medicine and Quality Assurance
;
: 39-47, 2014.
Article
in English
| WPRIM
| ID: wpr-218964
ABSTRACT
BACKGROUND:
Quantitative analysis of T-lymphocyte subsets is used to assess immune competency. Traditionally, T-lymphocyte subset analysis has been performed using flow cytometry, which requires complex instrumentation and relatively skilled manual operation. We evaluated the performance of an automated haematology analyser, the CELL-DYN Sapphire (CD Sapphire; Abbott Laboratories, USA) for T-lymphocyte subset analysis.METHODS:
The precision and linearity obtained using the CD Sapphire was evaluated. T-lymphocyte subsets in blood samples from 120 patients were quantified using CD Sapphire and flow cytometry (Cytomics FC 500; Beckman-Coulter, France). The time required for complete T cell subset analysis using both methods was also evaluated.RESULTS:
Results of CD Sapphire-based quantitation of CD3+, CD3+CD4+, and CD3+CD8+ cells showed intra-assay CV of less than 5% for precision and displayed linearity in the ranges of 84 to 5364, 41 to 2615, and 44 to 2800 cells/microL, respectively. There was good correlation among the CD3+, CD3+CD4+, and CD3+CD8+ cell counts as well as in the CD4/CD8 ratio (r=0.987, 0.982, 0.982, and 0.980, respectively) using CD Sapphire and flow cytometry. The mean turnaround time for the CD Sapphire (10.0+/-0.5 minutes) was significantly less than that for flow cytometry (111.8+/-8.4 minutes, P<0.001).CONCLUSIONS:
T cell subset analysis using the CD Sapphire gives excellent performance and consistent results that correlate well with those obtained by flow cytometry. We conclude that this time-efficient method can replace conventional flow cytometric methods used for measuring T cell subsets.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
CD4-Positive T-Lymphocytes
/
Cell Count
/
T-Lymphocyte Subsets
/
CD8-Positive T-Lymphocytes
/
Aluminum Oxide
/
Flow Cytometry
Limits:
Humans
Language:
English
Journal:
Journal of Laboratory Medicine and Quality Assurance
Year:
2014
Type:
Article
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