Serotype- and serogroup-specific detection of African horsesickness virus using phage displayed chicken scFvs for indirect double antibody sandwich ELISAs
Journal of Veterinary Science
;
: 95-98, 2013.
Article
in English
| WPRIM
| ID: wpr-219412
ABSTRACT
There is an ongoing need for standardized, easily renewable immunoreagents for detecting African horsesickness virus (AHSV). Two phage displayed single-chain variable fragment (scFv) antibodies, selected from a semi-synthetic chicken antibody library, were used to develop double antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISAs) to detect AHSV. In the DAS-ELISAs, the scFv previously selected with directly immobilized AHSV-3 functioned as a serotype-specific reagent that recognized only AHSV-3. In contrast, the one selected with AHSV-8 captured by IgG against AHSV-3 recognized all nine AHSV serotypes but not the Bryanston strain of equine encephalosis virus. Serving as evidence for its serogroup-specificity. These two scFvs can help to rapidly confirm the presence of AHSV while additional serotype-specific scFvs may simplify AHSV serotyping.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Vero Cells
/
Immunoglobulin G
/
Enzyme-Linked Immunosorbent Assay
/
Serologic Tests
/
Serotyping
/
Chlorocebus aethiops
/
Chickens
/
Peptide Library
/
African Horse Sickness Virus
/
Antibodies, Immobilized
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2013
Type:
Article
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