Survival of hypoxic human mesenchymal stem cells is enhanced by a positive feedback loop involving miR-210 and hypoxia-inducible factor 1
Journal of Veterinary Science
; : 69-76, 2013.
Article
in En
| WPRIM
| ID: wpr-219416
Responsible library:
WPRO
ABSTRACT
The use of mesenchymal stem cells (MSCs) has emerged as a potential new treatment for myocardial infarction. However, the poor viability of MSCs after transplantation critically limits the efficacy of this new strategy. The expression of microRNA-210 (miR-210) is induced by hypoxia and is important for cell survival under hypoxic conditions. Hypoxia increases the levels of hypoxia inducible factor-1 (HIF-1) protein and miR-210 in human MSCs (hMSCs). miR-210 positively regulates HIF-1alpha activity. Furthermore, miR-210 expression is also induced by hypoxia through the regulation of HIF-1alpha. To investigate the effect of miR-210 on hMSC survival under hypoxic conditions, survival rates along with signaling related to cell survival were evaluated in hMSCs over-expressing miR-210 or ones that lacked HIF-1alpha expression. Elevated miR-210 expression increased survival rates along with Akt and ERK activity in hMSCs with hypoxia. These data demonstrated that a positive feedback loop involving miR-210 and HIF-1alpha was important for MSC survival under hypoxic conditions.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Oxygen
/
Oxygen Consumption
/
Cell Survival
/
Gene Expression Regulation
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Cobalt
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MicroRNAs
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RNA, Small Interfering
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Hypoxia-Inducible Factor 1, alpha Subunit
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Mesenchymal Stem Cells
Limits:
Humans
Language:
En
Journal:
Journal of Veterinary Science
Year:
2013
Type:
Article