Down-regulation of survivin in growth inhibition of hepatoma cells induced by a selective cyclooxygenase-2 inhibitor / 대한간학회지
The Korean Journal of Hepatology
;
: 351-359, 2008.
Article
in Korean
| WPRIM
| ID: wpr-219567
ABSTRACT
BACKGROUND/AIMS:
Cyclooxygenase-2 (COX-2) inhibitors reportedly inhibit the growth of hepatocellular carcinoma (HCC) via caspase-dependent or caspase-independent apoptosis, which is due to COX-2 being associated with hepatocarcinogenesis. Survivin is highly expressed in most human cancers, but the mechanism regulating survivin expression remains unclear. We investigated the regulatory expression of survivin in selective-COX-2-inhibitor-induced growth inhibition of hepatoma cells.METHODS:
After treatment with NS-398 (a selective COX-2 inhibitor) at various concentrations (10, 50, 100, 150, and 200 micrometer), the growth inhibition of Hep3B hepatoma cells was assessed by an MTT cell-viability assay, DNA fragmentation gel analysis, and flow cytometry. The expression of survivin transcript was analyzed by reverse-transcription polymerase chain reactions.RESULTS:
NS-398 inhibited the growth of hepatoma cells by an amount dependent on the concentration and the time since treatment. Apoptotic DNA ladder and flow-cytometry shifting to the sub-G1 phase were revealed in NS-398-induced growth inhibition of hepatoma cells. NS-398 suppressed the expression of the survivin gene in a concentration- and time-dependent manner.CONCLUSIONS:
Survivin was down-regulated in the growth inhibition of hepatoma cells induced by a selective COX-2 inhibitor, NS-398, in a concentration- and time-dependent manner. These results suggest the therapeutic inhibition of COX-2 via suppression of survivin in HCC.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sulfonamides
/
Time Factors
/
G1 Phase
/
Carcinoma, Hepatocellular
/
Reverse Transcriptase Polymerase Chain Reaction
/
Cell Line, Tumor
/
Cell Proliferation
/
Cyclooxygenase 2 Inhibitors
/
Liver Neoplasms
/
Microtubule-Associated Proteins
Limits:
Humans
Language:
Korean
Journal:
The Korean Journal of Hepatology
Year:
2008
Type:
Article
Similar
MEDLINE
...
LILACS
LIS