Metacercarial proteins interacting with WD40-repeat protein of Clonorchis sinensis
The Korean Journal of Parasitology
;
: 229-232, 2007.
Article
in English
| WPRIM
| ID: wpr-219737
ABSTRACT
The WD40-repeat proteins serve as a platform coordinating partner proteins and are involved in a range of regulatory cellular functions. A WD40-repeat protein (CsWD1) of Clonorchis sinensis previously cloned is expressed stage-specifically in the tegumental syncytium of C. sinensis metacercariae. In the present study, interacting proteins with the CsWD1 protein was purified by immunoprecipitation and 2 dimension gel electrophoresis from the C. sinensis metacercaria soluble extract, and tryptic peptides were analyzed by LC/ESI-MS. Putative partner proteins were annotated to be actin-2, glyceraldehyde-3-phosphate dehydrogenase, and hypothetical and unmanned proteins. The CsWD1 protein was predicted to contain 3 conserved actin-interacting residues on its functional surface. With these results, the CsWD1 protein is suggested to be an actin-interacting protein of C. sinensis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Immunoglobulin G
/
Antibodies, Helminth
/
Electrophoresis, Gel, Two-Dimensional
/
Helminth Proteins
/
Clonorchis sinensis
/
Hydrogen-Ion Concentration
/
Microfilament Proteins
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2007
Type:
Article
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