The Combined Effect of Gamma Knife Irradiation and p53 Gene Transfection in Human Malignant Glioma Cell Lines
Journal of Korean Neurosurgical Society
;
: 48-53, 2005.
Article
in English
| WPRIM
| ID: wpr-220198
ABSTRACT
OBJECTIVE:
The purpose of this study is to elucidate in vitro responses to combined gamma knife irradiation and p53 gene transfection on human malignant glioma cell lines.METHODS:
Two malignant human glioma cell lines, U87MG (p53-wild type) and U373MG (p53-mutant) were transfected with an adenoviral vector containing p53 (MOI of 50) before and after applying 20Gy of gamma irradiation. Various assessments were performed, including, cell viability by MTT assay; apoptosis by annexin assay; and cell cycle by flow cytometry, for the seven groups mock, p53 only, gamma knife (GK) only, GK after LacZ, LacZ after GK, GK after p53, p53 after GK.RESULTS:
Cell survival decreased especially, in the subgroup transfected with p53 after gamma irradiation. Apoptosis tended to increase in p53 transfected U373 MG after gamma irradiation (apoptotic rate, 38.9%). The G2-M phase cell cycle arrest markedly increased by transfecting with p53, 48 hours after gamma knife irradiation in U373 MG (G2-M phase, 90.8%).CONCLUSION:
These results suggest that the in vitro effects of combined gamma knife irradiation and p53 gene transfection is an augmentation of apoptosis and G2-M phase cell cycle arrest, which are more exaggerated in U373 MG with p53 transfection after gamma knife irradiation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transfection
/
Genetic Therapy
/
Cell Cycle
/
Cell Line
/
Cell Survival
/
Adenoviridae
/
Genes, p53
/
Apoptosis
/
Cell Cycle Checkpoints
/
Flow Cytometry
Limits:
Humans
Language:
English
Journal:
Journal of Korean Neurosurgical Society
Year:
2005
Type:
Article
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