Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear
The Korean Journal of Parasitology
;
: 313-320, 2006.
Article
in English
| WPRIM
| ID: wpr-220307
ABSTRACT
In an effort to characterize, on the molecular scale, the Acanthamoeba initially isolated from the cornea of an amoebic keratitis patient associated with overnight-wear orthokeratology lens in Korea, we conducted mitochondrial DNA restriction fragment length polymorphism, 18S rDNA sequencing, and drug sensitivity analyses on the isolate (KA/PE1). The patient was treated with polyhexamethylene biguanide, chlorhexidine and oral itraconazole, which resulted in resolution of the patient's ocular inflammation. The majority of the molecular characteristics of the KA/PE1 were determined to be identical, or quite similar, to those of A. castellanii Ma strain, which had been isolated also from amoebic keratitis. The risk of Acanthamoeba keratitis as a potential complication of overnight orthokeratology is briefly discussed.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Astigmatism
/
Biguanides
/
Polymorphism, Restriction Fragment Length
/
DNA, Mitochondrial
/
DNA, Ribosomal
/
Acanthamoeba
/
RNA, Ribosomal, 18S
/
Acanthamoeba Keratitis
/
Chlorhexidine
/
DNA, Protozoan
Limits:
Animals
/
Female
/
Humans
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2006
Type:
Article
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