Cytotoxicity of temporary cements on bovine dental pulp-derived cells (bDPCs) using realtime cell analysis
The Journal of Advanced Prosthodontics
;
: 21-26, 2015.
Article
in English
| WPRIM
| ID: wpr-220558
ABSTRACT
PURPOSE:
To evaluate the cytotoxicity of temporary luting cements on bovine dental pulp-derived cells (bDPCs). MATERIALS ANDMETHODS:
Four different temporary cements were tested Rely X Temp E (3M ESPE), Ultratemp (Ultradent), GC Fuji Temp (GC), and Rely X Temp NE (3M ESPE). The materials were prepared as discs and incubated in Dulbecco's modified eagle's culture medium (DMEM) for 72 hours according to ISO 10993-5. A real-time cell analyzer was used to determine cell vitality. After seeding 200 microL of the cell suspensions into the wells of a 96-well plate, the bDPCs were cured with bioactive components released by the test materials and observed every 15 minutes for 98 hours. One-way ANOVA and Tukey-Kramer tests were used to analyze the results of the proliferation experiments.RESULTS:
All tested temporary cements showed significant decreases in the bDPCs index. Rely X Temp E, GC Fuji Temp, and Rely X Temp NE were severely toxic at both time points (24 and 72 hours) (P.05); however, the cell viability was significantly reduced at 72 hours (P<.001). Light and scanning electron microscopy examination confirmed these results.CONCLUSION:
The cytotoxic effects of temporary cements on pulpal tissue should be evaluated when choosing cement for luting provisional restorations.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Suspensions
/
Microscopy, Electron, Scanning
/
Cell Survival
Language:
English
Journal:
The Journal of Advanced Prosthodontics
Year:
2015
Type:
Article
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