Arg72Pro Polymorphism and Exon 7 Codon 249 Mutation of Plasma DNA p53 Gene in Early Hepatocellular Carcinoma Patients with Hepatitis B Virus Infection in a Korean population / 임상검사와정도관리
Journal of Laboratory Medicine and Quality Assurance
;
: 255-262, 2010.
Article
in English
| WPRIM
| ID: wpr-22069
ABSTRACT
BACKGROUND:
This study was performed to investigate on the genotypic frequencies of p53 Arg72Pro polymorphism and the prevalence of p53 codon 249 mutation in hepatocellular carcinoma patients.METHODS:
Plasma DNAs were extracted from the samples of 44 early HCC cases, 24 chronic B-viral hepatitis patients and 27 healthy individuals. Serum levels of AFP, PIVKA-II, and HBV DNA-positive rates among the study groups were also compared. PCR-based restriction fragment length polymorphism method was used to determine p53 Arg72Pro genotype and to detect codon 249 mutation.RESULTS:
Serum AFP and PIVKA-II level, Edmondson grade, tumor size and frequency of HBV DNA-positivity among HCC group according to Arg72Pro genotypes showed no statistically significant difference. The frequencies of Arg72Pro genotypes (Arg/Arg, Arg/Pro, Pro/Pro) were respectively as follows 34.1%, 47.7%, 18.2% in HCC group; 29.2%, 54.2%, 16.7% in hepatitis group; 29.6%, 55.6%, 14.8% in control group. Pro homozygote genotype had a higher risk for developing HCC by adjusted OR (1.529, 95% CI 0.325-7.193), but not statistically significant (P=0.591). No codon 249 mutation was found among 44 HCC cases.CONCLUSIONS:
Pro homozygote was around 16% in all study groups, and did not statistically increase risks to developing HCC. We suggest that Arg72Pro polymorphism of p53 gene is not a significant risk factor in early hepatocarcinogenesis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasma
/
Protein Precursors
/
Polymorphism, Restriction Fragment Length
/
Codon
/
DNA
/
Prothrombin
/
Biomarkers
/
Hepatitis B virus
/
Exons
/
Prevalence
Type of study:
Etiology study
/
Prevalence study
/
Risk factors
Limits:
Humans
Language:
English
Journal:
Journal of Laboratory Medicine and Quality Assurance
Year:
2010
Type:
Article
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