Retinoic Acid-induced Differentiation of Rat Mesenchymal Stem Cells into beta-Cell Lineage / 대한이식학회지
The Journal of the Korean Society for Transplantation
;
: 118-129, 2015.
Article
in English
| WPRIM
| ID: wpr-220923
ABSTRACT
BACKGROUNDS Type I diabetes mellitus (T1DM), an autoimmune disease, is associated with insulin deficiency due to the death of beta-cells. Bone marrow-derived mesenchymal stem cells (BM-MSCs) are capable of tissue repair and thus are a promising source of beta-cell surrogates. METHODS:
In this study, the therapeutic potential of BM-MSCs as beta-cell replacements was analyzed both in vitro and in vivo. First, we used retinoic acid (RA) to induce rat BM-MSCs to differentiate into cells of endodermal/pancreatic lineage. Then, differentiated rat BM-MSCs were syngeneically injected under the renal capsule of rats.RESULTS:
Analysis of gene expression revealed that rat BM-MSCs showed signs of early pancreatic development, and differentiated cells were qualitatively and quantitatively confirmed to produce insulin in vitro. In vivo study was performed for short-term (3 weeks) and long-term (8 weeks) period of time. Rats that were injected with differentiated MSCs exhibited a reduction in blood glucose levels throughout 8 weeks, and grafted cells survived in vivo for at least 3 weeks.CONCLUSIONS:
These findings show that RA can induce differentiation of MSCs into the beta-cell lineage and demonstrate the potential of BM-MSCs to serve as therapeutic tools for T1DM.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Autoimmune Diseases
/
Tretinoin
/
Blood Glucose
/
Gene Expression
/
Transplants
/
Diabetes Mellitus
/
Diabetes Mellitus, Type 1
/
Insulin-Secreting Cells
/
Mesenchymal Stem Cells
/
Insulin
Limits:
Animals
Language:
English
Journal:
The Journal of the Korean Society for Transplantation
Year:
2015
Type:
Article
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