Effect of Glutathione on Oxidant-induced Cardiotoxicity
Korean Circulation Journal
;
: 433-437, 1997.
Article
in Korean
| WPRIM
| ID: wpr-22126
ABSTRACT
BACKGROUND:
In order to elucidate toxic mechanism of the oxygen radicals on cultured rat myocardial cells, cytotoxic effect of oxygen radicals was evaluated by MTT assay. In addition protective effect of glutathione(GSH) on oxidant-induced cardiotoxicity was investigated on these cultures.METHODS:
Myocardial cells derived from neonatal rats were cultured for 12 hours in the medium containing various concentrations of glucose oxidase(GO). Cell viability was measured by MTT assay and morphological changes of the myocardial cells were observed by light microscope.RESULTS:
GO-mediated oxygen radicals remarkably decreased cell viability of cultured myocardial cells in a dose-and time-dependent manner. And also, GSH blicked GO-induced cardiotoxicity in these cultures.CONCLUSION:
These results suggest that the oxygen radicals are tixic and the selective antioxidants such as GSH are effective in blocking against the oxidant-induced cardiotoxicity in cultures of the myocardial cells of neonatsl rats.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cell Survival
/
Reactive Oxygen Species
/
Glucose
/
Glutathione
/
Antioxidants
Limits:
Animals
Language:
Korean
Journal:
Korean Circulation Journal
Year:
1997
Type:
Article
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