Simultaneous HPLC Analysis of Three Flavonoids in the Extracts of Artocarpus heterophyllus Heartwoods
Natural Product Sciences
;
: 77-81, 2016.
Article
in English
| WPRIM
| ID: wpr-221639
ABSTRACT
A reversed-phase high-performance liquid chromatographic method is described for the simultaneous determination of three antibacterial flavonoids, artocarpanone, artocarpin, and cycloartocarpin in ethyl acetate extracts from Artocarpus heterophyllus heartwoods. Separation was achieved using a TSK-gel ODS-80Tm column (5 µm, 4.6 × 150 mm) at 25℃ with a gradient elution system of methanol and water as follows 0-8 min, 6040; 8-27 min, 8020; 27-35 min, 6040, v/v, at a flow rate of 1 mL/min, and a quantitative UV detection at 285 nm. The method was validated by measuring the key parameters, including specificity, linearity, sensitivity, accuracy, repeatability and reproducibility. A high degree of specificity and sensitivity was achieved. The calibration curves for all three flavonoids showed good linearity with a coefficient of determinations (R²) of ≥ 0.9995. The recoveries of the method were from 98-104%, with good reproducibility and repeatability (RSD values of less than 2%) were also achieved. Ethyl acetate was the best solvent for extraction of these three flavonoids using the heat reflux conditions for 1 h. This optimized sample preparation and HPLC method can be practically used for a routine standardization process of the extracts from the A. heterophyllus heartwoods.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Flavonoids
/
Calibration
/
Water
/
Chromatography, High Pressure Liquid
/
Sensitivity and Specificity
/
Artocarpus
/
Methanol
/
Hot Temperature
/
Methods
Type of study:
Diagnostic study
Language:
English
Journal:
Natural Product Sciences
Year:
2016
Type:
Article
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