Induction of Prostaglandin E₂ by Porphyromonas gingivalis in Human Dental Pulp Cells
International Journal of Oral Biology
;
: 149-153, 2017.
Article
in English
| WPRIM
| ID: wpr-222405
ABSTRACT
Cyclooxygenase-2 (COX-2)-mediated prostaglandin E₂ (PGE₂) plays a key role in development and progression of inflammatory responses and Porphyromonas gingivalis is a common endodontic pathogen. In this study, we investigated induction of COX-2 and PGE₂ by P. gingivalis in human dental pulp cells (HDPCs). P. gingivalis increased expression of COX-2, but not that of COX-1. Increased levels of PGE₂ were released from P. gingivalis-infected HDPCs and this PGE₂ increase was blocked by celecoxib, a selective COX-2 inhibitor. P. gingivalis activated all three types of mitogen-activated protein kinases (MAPKs). P. gingivalis-induced activation of nuclear factor-κB (NF-κB) was demonstrated by the results of phosphorylation of NF-κ B p65 and degradation of inhibitor of κB-α (IκB-α). Pharmacological inhibition of each of the three types of MAPKs and NF-κB substantially attenuated P. gingivalis induced PGE2 production. These results suggest that P. gingivalis should promote endodontic inflammation by stimulating dental pulp cells to produce PGE₂.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphorylation
/
Pulpitis
/
Dinoprostone
/
Porphyromonas
/
Porphyromonas gingivalis
/
Mitogen-Activated Protein Kinases
/
Dental Pulp
/
Cyclooxygenase 2
/
Celecoxib
Limits:
Humans
Language:
English
Journal:
International Journal of Oral Biology
Year:
2017
Type:
Article
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