CD8+ T-cell Activation in Mice Injected with a Plasmid DNA Vaccine Encoding AMA-1 of the Reemerging Korean Plasmodium vivax
The Korean Journal of Parasitology
;
: 85-90, 2011.
Article
in English
| WPRIM
| ID: wpr-222442
ABSTRACT
Relatively little has been studied on the AMA-1 vaccine against Plasmodium vivax and on the plasmid DNA vaccine encoding P. vivax AMA-1 (PvAMA-1). In the present study, a plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax has been constructed and a preliminary study was done on its cellular immunogenicity to recipient BALB/c mice. The PvAMA-1 gene was cloned and expressed in the plasmid vector UBpcAMA-1, and a protein band of approximately 56.8 kDa was obtained from the transfected COS7 cells. BALB/c mice were immunized intramuscularly or using a gene gun 4 times with the vaccine, and the proportions of splenic T-cell subsets were examined by fluorocytometry at week 2 after the last injection. The spleen cells from intramuscularly injected mice revealed no significant changes in the proportions of CD8+ T-cells and CD4+ T-cells. However, in mice immunized using a gene gun, significantly higher (P<0.05) proportions of CD8+ cells were observed compared to UB vector-injected control mice. The results indicated that cellular immunogenicity of the plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax was weak when it was injected intramuscularly; however, a promising effect was observed using the gene gun injection technique.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmodium vivax
/
Lymphocyte Activation
/
Protozoan Proteins
/
Chlorocebus aethiops
/
Protozoan Vaccines
/
Malaria, Vivax
/
CD8-Positive T-Lymphocytes
/
COS Cells
/
Vaccines, DNA
/
Membrane Proteins
Limits:
Animals
/
Humans
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2011
Type:
Article
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