Identification of Antigenic Proteins in Trichomonas vaginalis
The Korean Journal of Parasitology
;
: 79-83, 2011.
Article
in English
| WPRIM
| ID: wpr-222443
ABSTRACT
Trichomoniasis is a sexually transmitted disease due to infection with Trichomonas vaginalis, and it can cause serious consequences for women's health. To study the virulence factors of this pathogen, T. vaginalis surface proteins were investigated using polyclonal antibodies specific to the membrane fractions of T. vaginalis. The T. vaginalis expression library was constructed by cloning the cDNA derived from mRNA of T. vaginalis into a phage lambda Uni-ZAP XR vector, and then used for immunoscreening with the anti-membrane proteins of T. vaginalis antibodies. The immunoreactive proteins identified included adhesion protein AP65-1, alpha-actinin, kinesin-associated protein, teneurin, and 2 independent hypothetical proteins. Immunofluorescence assays showed that AP65-1, one of the identified immunogenic clones, is prevalent in the whole body of T. vaginalis. This study led us to identify T. vaginalis proteins which may stimulate immune responses by human cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Trichomonas Infections
/
Trichomonas vaginalis
/
Molecular Sequence Data
/
Protozoan Proteins
/
Antigens, Protozoan
Type of study:
Prognostic study
Limits:
Animals
/
Female
/
Humans
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2011
Type:
Article
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