Evaluation of Propidium Monoazide Real-Time PCR for Viablity of Mycobacterium leprae / 나학회지
Korean Leprosy Bulletin
;
: 13-22, 2016.
Article
in Korean
| WPRIM
| ID: wpr-222629
ABSTRACT
BACKGROUND:
Conventional acid-fast bacilli (AFB) staining cannot differentiate viable from dead cells. Propidium monoazide (PMA) is a photoreactive DNA-binding dye that inhibits PCR amplification by DNA modification. OBJECT The author evaluated whether PMA real-time PCR is suitable for the viablity of Mycobacterium leprae (M. leprae) in specimens of cultivation in mouse foot pads.METHODS:
A total of 55 diluted suspensions from mouse foot pads were quadruplicated and subjected to PMA treatment and/or heat inactivation, and were also tested to compare the ΔCT values (CT value in PMA-treated samples-CT value in non-PMA-treated samples). Real-time PCR was performed using QuantiTect SYBR® Green PCR Kits(Qiagen, USA), and the CT value changes after PMA treatment were compared between PMA treatment and/or heat inactivation groups.RESULTS:
The increase in the CT value after PMA treatment was significant in heat inactivated group(4.26) and non-heat inactivated group(1.12)(both P = 0.000). In the ROC curve analysis, the cutoff ΔCT value for maximum sensitivity (100%) and specificity (97.1%) for differentiating dead from live cells was 2.41CONCLUSIONS:
PMA real-time PCR is a useful approach for evaluating viablity of M. leprae.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Propidium
/
Suspensions
/
DNA
/
Polymerase Chain Reaction
/
ROC Curve
/
Sensitivity and Specificity
/
Real-Time Polymerase Chain Reaction
/
Foot
/
Hot Temperature
/
Mycobacterium
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
Korean
Journal:
Korean Leprosy Bulletin
Year:
2016
Type:
Article
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