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Expression analysis of ciliary rootlet coiled coil protein mRNA during Xenopus development / 대한수의학회지
Korean Journal of Veterinary Research ; : 181-184, 2015.
Article in English | WPRIM | ID: wpr-223843
ABSTRACT
Ciliary rootlet coiled coil protein (CROCC), the structural component that originates from the basal body at the proximal end of the ciliary rootlet, plays a crucial role in maintaining the cellular integrity of ciliated cells. In the current study, we cloned Xenopus CROCC and performed the expression analysis. The amino acid sequence of Xenopus laevis was related to those of Drosophila, cow, goat, horse, chicken, mouse and human. Reverse transcription polymerase chain reaction analysis revealed that CROCC mRNA encoding a coiled coil protein was present maternally, as well as throughout early development. In situ hybridization indicated that CROCC mRNA occurred in the animal pole of embryo during gastrulation and subsequently in the presumptive neuroectoderm at the end of gastrulation. At tailbud stages, CROCC mRNA expression was localized in the anterior roof plate of the developing brain, pharyngeal epithelium connected to gills, esophagus, olfactory placode, intestine and nephrostomes of the pronephric kidney. Our study suggests that CROCC may be responsible for control of the development of various ciliated organs.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Xenopus / Xenopus laevis / Brain / RNA, Messenger / Goats / Chickens / Polymerase Chain Reaction / Amino Acid Sequence / Clone Cells / In Situ Hybridization Limits: Animals / Humans Language: English Journal: Korean Journal of Veterinary Research Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Xenopus / Xenopus laevis / Brain / RNA, Messenger / Goats / Chickens / Polymerase Chain Reaction / Amino Acid Sequence / Clone Cells / In Situ Hybridization Limits: Animals / Humans Language: English Journal: Korean Journal of Veterinary Research Year: 2015 Type: Article