Establishment and characterization of secondary drug resistant cell line originating from cisplatin-resistant SNU-8 / 대한산부인과학회지
Korean Journal of Obstetrics and Gynecology
;
: 101-110, 2007.
Article
in Korean
| WPRIM
| ID: wpr-224172
ABSTRACT
OBJECTIVE:
Multidrug resistance to chemotherapy is a major obstacle in attempts to improve the clinical outcome of ovarian carcinoma patients. The aim of this study is to establish secondary anticancer drug resistant cell line from original SNU-8/WT resistant to cisplatin and characterize it.METHODS:
After establishing secondary drug resistant cell line (SNU-8/Fac), drug sensitivity was measured by MTT assay. Multidrug resistance (MDR) was analyzed by RT-PCR and western blotting analysis.RESULTS:
MTT assay data demonstrated that MDR was expressed in SNU-8/Fac. In addition, mRNA expression of MDR1, ATP7B in SNU-8/Fac was increased. However, overexpression of MRP1, BCRP, TS and MT mRNA was not observed. At the protein level, protein P-gp, ATP7B were overexpressed in SNU-8/Fac.CONCLUSION:
We established a new anticancer drug resistant cell line from original SNU-8/WT. Overexpression of P-gp and ATP7B was observed in SNU-8/Fac.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ovarian Neoplasms
/
RNA, Messenger
/
Cell Line
/
Blotting, Western
/
Cisplatin
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Drug Resistance, Multiple
/
Drug Therapy
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2007
Type:
Article
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