Ginkgolide B Modulates BDNF Expression in Acute Ischemic Stroke
Journal of Korean Neurosurgical Society
;
: 391-396, 2017.
Article
in English
| WPRIM
| ID: wpr-224196
ABSTRACT
OBJECTIVE:
To investigate the neuroprotective effects of Ginkgolide B (GB) against ischemic stroke-induced injury in vivo and in vitro, and further explore the possible mechanisms concerned.METHODS:
Transient middle cerebral artery occlusion (tMCAO) mice and oxygen-glucose deprivation/reoxygenation (OGD/R)-treated N2a cells were used to explore the neuroprotective effects of GB. The expression of brain-derived neurotrophic factor (BDNF) was detected via Western blot and qRT-PCR.RESULTS:
GB treatment (4 mg/kg, i. p., bid) significantly reduced neurological deficits, water content, and cerebral infarct volume in tMCAO mice. GB also significantly increased Bcl-2/Bax ratio, reduced the expression of caspase-3, and protected against OGD/R-induced neuronal apoptosis. Meanwhile, GB caused the up-regulation of BDNF protein in vivo and in vitro.CONCLUSION:
Our data suggest that GB might protect the brain against ischemic insult partly via modulating BDNF expression.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
In Vitro Techniques
/
Brain
/
Water
/
Up-Regulation
/
Blotting, Western
/
Apoptosis
/
Neuroprotective Agents
/
Brain-Derived Neurotrophic Factor
/
Stroke
/
Infarction, Middle Cerebral Artery
Limits:
Animals
Language:
English
Journal:
Journal of Korean Neurosurgical Society
Year:
2017
Type:
Article
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