Expression of Recombinant Porcine Interleukin-2 and Application of Its Antibody to Immunoassays
Journal of Veterinary Science
;
: 207-212, 2002.
Article
in English
| WPRIM
| ID: wpr-22471
ABSTRACT
Interleukin-2 plays an important role in T lymphocyte proliferation and immune response regulations. In this study, porcine IL-2 cDNA was cloned from peripheral blood mononuclear cells, and recombinant porcine IL-2 (rpIL-2) was expressed in Escherichia coli. The size of rpIL-2 without signal peptides was about 15 kDa when determined by SDS-PAGE and Western blotting analysis. Anti-rpIL-2 antibody was produced from mice immunized with the purified rpIL-2, and its specificity was examined by Western blotting and ELISA. In the Western blotting assay, anti-rpIL-2 and anti-recombinant human IL-2 (rhIL-2) antibodies specifically recognized rpIL-2 and rhIL-2, respectively. However, anti-rpIL-2 antibody did not recognize rhIL-2, and anti-rhIL-2 antibody also did not react with rpIL-2 in the same assay. In ELISA, anti-rpIL-2 antibody strongly interacted with both rpIL-2 and rhIL-2, and anti-rhIL-2 antibody also efficiently recognized both proteins. Taken together, the specificity of anti-rpIL-2 antibody for rpIL-2 was demonstrated by Western blotting and ELISA. It was also shown that ELISA is more efficient than Western blotting in determining the species cross-reactivity of anti-rpIL-2 antibody.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Species Specificity
/
Swine
/
Recombinant Proteins
/
Enzyme-Linked Immunosorbent Assay
/
Blotting, Western
/
Interleukin-2
/
Cloning, Molecular
/
Cross Reactions
/
Electrophoresis, Polyacrylamide Gel
/
Escherichia coli
Limits:
Animals
/
Humans
Language:
English
Journal:
Journal of Veterinary Science
Year:
2002
Type:
Article
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