Increased Growth of Human Leukemic HL-60 Cells by Dimethyl Sulfoxide and Dimethylformamide / 대한면역학회지
Korean Journal of Immunology
;
: 285-289, 1999.
Article
in Korean
| WPRIM
| ID: wpr-224752
ABSTRACT
A recent study has proposed that dimethyl sulfoxide (DMSO) increases the number of HL-60 cells in high cell density conditions by inhibiting cell density-dependent apoptosis. We observed that dimethylformamide (DMF), a DMSO-related polar compound, also increased the concentration of HL-60 cells. The effective dose range of DMSO and DMF was 0.5-1% and 0.2-0.6% respectively. DMF, like DMSO, inhibited density-dependent apoptosis of HL-60 cells. The flow cytometric PKH26 cell proliferation assay showed DMSO and DMF actively increased cell division. However, the difference in the distribution of cell cycle phase was not noted between the control and the DMSO- or DMF-treated HL-60 cultures. Finally, DMSO and DMF stimulated HL-60 growth even in low density conditions. These results suggest that DMSO and OMF at appropriate concentrations increase the number of HL-60 cells by both apoptosis inhibition and cell division augmentation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cell Count
/
Cell Cycle
/
Cell Division
/
Dimethyl Sulfoxide
/
Apoptosis
/
HL-60 Cells
/
Cell Proliferation
/
Dimethylformamide
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Immunology
Year:
1999
Type:
Article
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