Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak
Journal of Korean Medical Science
;
: 315-320, 2016.
Article
in English
| WPRIM
| ID: wpr-225574
ABSTRACT
During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Vero Cells
/
RNA, Viral
/
Microscopy, Electron
/
Chlorocebus aethiops
/
Polymerase Chain Reaction
/
Disease Outbreaks
/
Sequence Analysis, RNA
/
Coronavirus Infections
/
Republic of Korea
Limits:
Animals
/
Humans
Country/Region as subject:
Asia
Language:
English
Journal:
Journal of Korean Medical Science
Year:
2016
Type:
Article
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