Effect of p16 on glucocorticoid response in a B-cell lymphoblast cell line / 소아과
Korean Journal of Pediatrics
;
: 753-758, 2010.
Article
in English
| WPRIM
| ID: wpr-225657
ABSTRACT
PURPOSE:
It has been suggested that p16 has a role in glucocorticoid (GC)-related apoptosis in leukemic cells, but the exact mechanisms have yet to be clarified. We evaluated the relationship between the GC response and p16 expression in a lymphoma cell line.METHODS:
We used p16 siRNA transfection to construct p16-inactivated cells by using the B-cell lymphoblast cell line NC-37. We compared glucocorticoid receptor (GR) expression, apoptosis, and cell viability between control (p16+ NC-37) and p16 siRNA-transfected (p16- NC-37) cells after a single dose of dexamethasone (DX).RESULTS:
In both groups, there was a significant increase in cytoplasmic GR expression, which tended to be higher for p16+ NC-37 cells than for p16- NC37 cells at all times, and the difference at 18 h was significant (P<0.05). Similar patterns of early apoptosis were observed in both groups, and late apoptosis occurred at higher levels at 18 h when the GR had already been downregulated (P<0.05). Cell viability decreased in both groups but the degree of reduction was more severe in p16+ NC-37 cells after 18 h (P<0.05).CONCLUSION:
These results suggest a relationship between GR expression and cell cycle inhibition, in which the absence of p16 leads to reduced cell sensitivity to DX.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Dexamethasone
/
B-Lymphocytes
/
Transfection
/
Receptors, Glucocorticoid
/
Cell Cycle
/
Cell Line
/
Cell Survival
/
Apoptosis
/
Cytoplasm
/
RNA, Small Interfering
Language:
English
Journal:
Korean Journal of Pediatrics
Year:
2010
Type:
Article
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