Comparision in the yield of fetal nucleated red blood cell between the first-and second-trimester using double density gradient centrifugation / 대한산부인과학회지
Korean Journal of Obstetrics and Gynecology
;
: 127-136, 2010.
Article
in English
| WPRIM
| ID: wpr-22600
ABSTRACT
OBJECTIVE:
The aim of our study was to make a practical comparative evaluation of the first and second trimesters in order to determine the period during which a higher yield of fetal nucleated red blood cells (FNRBCs) can be obtained.METHODS:
NRBCs were isolated from maternal blood during the first and second trimesters of pregnancy using double Percoll gradients with different osmolarities. Magnetic activated cell sorting was performed with Kleihauer-Betke stain. We isolated fetal NRBCs from 10 mL of samples of maternal blood and determined fetal sex and fetal aneuploidy by fluorescence in situ hybridization (FISH).RESULTS:
The average number of NRBCs was 9.85 in samples obtained during the first trimester and 14.88 in samples obtained during the second trimester (P=0.07). The average number of NRBCs with Y chromosome signals was 5.73 in the first trimester and 8.22 in second trimester (P=0.56). However, the percentage of NRBCs with Y chromosome signals in the first trimester (70.6%) was significantly higher than in the second trimester (59.8%) (P=0.049). We diagnosed the blood samples from 7 pregnant women having fetal aneuploidy using this method and the number of NRBCs was 18.4.CONCLUSION:
The method using Percoll osmolarity and a double density gradient system may be a very useful method for separation of NRBCs in the first trimester of pregnancy and also in the second trimester.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osmolar Concentration
/
Pregnancy Trimester, Second
/
Pregnancy Trimester, First
/
Prenatal Diagnosis
/
Y Chromosome
/
Centrifugation, Density Gradient
/
Povidone
/
In Situ Hybridization
/
Silicon Dioxide
/
Pregnant Women
Type of study:
Diagnostic study
Limits:
Female
/
Humans
/
Pregnancy
Language:
English
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2010
Type:
Article
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