T Cell Immunoglobulin Mucin Domain (TIM)-3 Promoter Activity in a Human Mast Cell Line
Immune Network
;
: 207-212, 2012.
Article
in English
| WPRIM
| ID: wpr-226024
ABSTRACT
T cell immunoglobulin mucin domain (TIM)-3 is an immunomodulatory molecule and upregulated in T cells by several cytokines. TIM-3 also influences mast cell function but its transcriptional regulation in mast cells has not been clarified. Therefore, we examined the transcript level and the promoter activity of TIM-3 in mast cells. The TIM-3 transcript level was assessed by real-time RT-PCR and promoter activity by luciferase reporter assay. TIM-3 mRNA levels were increased in HMC-1, a human mast cell line by TGF-beta1 stimulation but not by stimulation with interferon (IFN)-alpha, IFN-lambda, TNF-alpha, or IL-10. TIM-3 promoter -349~+144 bp region relative to the transcription start site was crucial for the basal and TGF-beta1-induced TIM-3 promoter activities in HMC-1 cells. TIM-3 promoter activity was increased by overexpression of Smad2 and Smad4, downstream molecules of TGF-beta1 signaling. Our results localize TIM-3 promoter activity to the region spanning -349 to +144 bp in resting and TGF-beta1 stimulated mast cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Immunoglobulins
/
RNA, Messenger
/
T-Lymphocytes
/
Cytokines
/
Interferons
/
Tumor Necrosis Factor-alpha
/
Interleukin-10
/
Transcription Initiation Site
/
Transforming Growth Factor beta1
/
Luciferases
Limits:
Humans
Language:
English
Journal:
Immune Network
Year:
2012
Type:
Article
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